Rice University

Shared Equipment Authority

BioSEA

Microscope: Nikon A1-Rsi Confocal

Nikon A1-Rsi is a confocal system equipped with a unique hybrid confocal scan head with 5 highly sensitive PMT (4-fluorescences and 1-transmitted light) detectors and a 32-channel PMT spectral detector for 400-750nm bandwith. The system allows increased light detection efficiency and has a standard high-resolution confocal galvano mode (up to 4096 x 4096 pixels) as well as a high speed resonant mode capable of scanning 420 fps at 512 x 32 pixels (30fps at 512x512 pixels).

Imaging Software: NIS Element PC Off-line

The full version of Nikon NIS-Element C software for image processing and analysis (including 2D/3D cell tracker) except microscope controller. It works for any TIFF files exported from other platforms such as Zeiss (Zen, AxioImager & LSM), Olympus and Leica.

Adobe Photoshop, Microsoft Office, NIH Image J and FIJI are also installed in the system.

The Desktop based on Dell Precision T3600 with Windows professional 64-bit using Four Core XEON 3.6 GHz and 16GB DDR3 RAM. It has Dual 1GB AMD Video Card and a total of 2 GB RAID HD and 30" HD monitor (2560x1600 pixels)."