Rice University

Shared Equipment Authority

Microscopes

Microscope: Zeiss Axioimager Z2

Zeiss Axio Imager Z2 upright microscope is capable to perform epi-fluorescence imaging in combination with transmitted-light brightfield, darkfield and DIC. The system is equipped with series of objectives (2.5X, 5X, 10X, 20X, 40x dry and 63X, 100X oil) with complete DIC sliders and corresponding modules/analyzers. The new 63X water dipping objective just recently purchased (Dec 2013). It also has mechanical stage 75x50R and heavy duty focusing drive motorized (for x,y,z movement) with 10 nm stepsize.

Microscope: Nikon A1-Rsi Confocal

Nikon A1-Rsi is a confocal system equipped with a unique hybrid confocal scan head with 5 highly sensitive PMT (4-fluorescences and 1-transmitted light) detectors and a 32-channel PMT spectral detector for 400-750nm bandwith. The system allows increased light detection efficiency and has a standard high-resolution confocal galvano mode (up to 4096 x 4096 pixels) as well as a high speed resonant mode capable of scanning 420 fps at 512 x 32 pixels (30fps at 512x512 pixels).