Nikon A1-Rsi is a confocal system equipped with a unique hybrid confocal scan head with 5 highly sensitive PMT (4-fluorescences and 1-transmitted light) detectors and a 32-channel PMT spectral detector for 400-750nm bandwith. The system allows increased light detection efficiency and has a standard high-resolution confocal galvano mode (up to 4096 x 4096 pixels) as well as a high speed resonant mode capable of scanning 420 fps at 512 x 32 pixels (30fps at 512x512 pixels).
The system is mounted on a Nikon Ti-E widefield, inverted, fluorescence microscope with DIC/Phase of 10X, 20X, 40X and 60x dry/oil/water immersion optics. It has 7 laser lines, comprises of diode lasers (for 405 nm, 561nm and 640 nm) and argon multi laser lines (for 457/477/488/514nm). Enhanced Spectral Imaging allows fast spectral imaging at 16 fps (512 x 64 pixels) and real-time Spectral Unmixing.
The Nikon A1-Rsi is designed for live cell imaging using Okolab environmental chamber to regulate temperature and air/CO2/N2. It has Nikon’s Perfect Focus System (PFS) to maintain focus on sample by compensating for thermal drifts and Prior motorized stage for tiling large areas and multi-point mark and find (high-content imaging) with various stage adapters for different type of petri-dishes, slides and multi-well plates. The system can also be used for Reflectance Confocal Microscopy technique.
Nikon NIS Elements C imaging software allows users to perform real-time FRAP, FLIP, FRET, calcium imaging (with appropriate dyes), photo activation/stimulation, high-content and time-lapse live cell imaging as well as data management and various analysis. It also works with TIFF-exported files from other platforms such as Zeiss ZEN/LSM, Leica or Olympus.
A separate off-line desktop version of NIS-Element for image analysis and processing can be booked at lower fee.