Fluorescence based quantitative analysis of cells or bacteria
Budi Utama - firstname.lastname@example.org
Flow cytometer with 2-laser, 6-color (4-2) configuration. Uses blue (488 nm) and red (633 nm) lasers to excite fluorescent objects in the flow cell. FACSDiva software provides quantification of cell populations, based on fluorescence emission and forward (FSC) and side (SSC) scattering, related to cell size and shape (granularity). FlowJo software is also available on the workstation for advanced data analysis.
Online training resources are available from BD Biosciences here.
Also, we now have access to FluoroFinder, a free online resource to help users choose appropriate fluorophores, and design sample panels. A link to our instrument, set up on their site, is here, and the general site they set up for Rice University is here.